- T. cruzi B13: Chagas disease is an infectious disease caused by a protozoan parasite (Trypanosoma cruzi). About 6–7 million people worldwide are estimated to be infected with T. cruzi, leading to approximately 12 000 deaths every year. Chagas disease is found mainly in endemic areas of 21 continental Latin American countries, where transmission is largely related to the vector presence. Today, approximately 75 million people are considered at risk of infection. Up to a third of people with chronic infection develop cardiac alterations and 1 in 10 develop digestive, neurological or mixed alterations which may require specific treatment. T.cruzi-B13 is Cytoplasmic/surface antigen, an immunodominant recombinant antigen which encodes part of the tandemly repeated domain of the T. cruzi 140/116kDa protein located on the surface of infective trypomastigotes. B13 protein is recognized by IgG serum antibodies from 97% of T. cruzi infected individuals 2 Journal of Parasitology Research in Latin America, bearing both forms of chronic Chagas’ disease: chronic Chagas’ diseased cardiomyopathy (CCC) and the asymptomatic/indeterminate form. Recombinant T.cruzi-B13 contains 227 amino acids with C terminal his tag, the protein migrates on SDS-PAGE with 30kDa in size and over 95% purity.
2. Orov-NP: Oropouche virus is transmitted primarily by biting midges and some mosquitoes. Oropouche virus is endemic to the Amazon basin in South America, Central America, and the Caribbean. Oropouche virus belongs to arboviral disease. The virus was first detected in 1955 in Trinidad and Tobago near the Oropouche River.
Oropouche virus (OROV) starts with the abrupt onset of fever, headache (often severe), chills, myalgia, and arthralgia, 4% cases complicated with neuroinvasive disease (e.g., meningitis and encephalitis). Infections during pregnancy have been associated with fetal death and potential birth defects. Due to its symptom overlap with other viruses like Dengue, Zika, Chikungunya, and Yellow Fever, early and accurate diagnostics are critical for identifying OROV.
Recombinant Orov-NP is developed with 240 amino acids with C terminal His tag and 95% in purity, it migrates on SDS-PAGE showing 25kD in size.